Spectrasol Applications
Sample Type: Powdered & Liquid Milk
|
 Preparation: |
Prepare a 10% aqueous solution of the Spectrasol CFA-C reagent
and adjust its pH to 8 with re-distilled HNO3.
Dissolve 0.1 gm of milk powder (or 1.0 mls liquid milk) in 10.0
mls (or 9.0mls) of 10% Spectrasol CFA-C adjusted to pH 8.
Use rinse solution of 3% (v/v) ammonia after every two samples
to prevent clogging.
|
| Results: |
Comparison of ICP results (n = 4) using a 10% solution of the
Spectrasol CFA-C reagent to NIST 1549 non-fat milk powder SRM. |
|
| Analyte |
ICP Analysis |
NIST Certified |
Recovery |
|
(%) |
(%) |
(%) |
|
| Ca |
1.22±0.02 |
1.30±0.05 |
94 |
| K |
1.62±0.02 |
1.69±0.03 |
96 |
| Mg |
0.112±0.003 |
0.120±0.003 |
93 |
| Na |
0.464±0.009 |
0.497±0.010 |
93 |
| P |
1.02±0.02 |
1.06±0.02 |
96 |
| Zn |
45.3±0.8 |
46.1±2.2 |
98 |
|
| Discussion: |
ICP-MS analysis was also used where the samples were prepared
as 0.2gm of milk powder (or 2.5 mls liquid milk) dissolved in
49.5 mls (or 47.0 mls) 5% CFA-C solution adjusted to pH 8.
|
| Reference: |
Nobrega, J.A. et al, Direct determination of major and minor trace
elements in milk by ICP and ICP-MS. J. Anal. At. Spectrom., 12,000
(1997). |